CD19+CD24hiCD27+调节性B细胞在人布鲁菌病中的变化和作用

贾?斌; 弋中涛; 伊帕尔古丽·亚森; 梁思敏; 高?辉

文章摘要

CD19+CD24hiCD27+调节性B细胞在人布鲁菌病中的变化和作用

Changes and roles ofCD19+CD24hiCD27+ regulatory B cells in brucellosis

DOI：10.3969/j.issn.1007-8134.2023.04.10

中文关键词: 布鲁菌病调节性B细胞细胞因子免疫反应

英文关键词: brucellosis regulatory B cells cytokines immune response

基金项目:新疆维吾尔自治区自然科学基金面上项目（2019D01C294）

作者	单位
贾?斌	新疆医科大学第一附属医院呼吸科
弋中涛	新疆医科大学第八附属医院布病科
伊帕尔古丽·亚森	新疆医科大学第八附属医院布病科
梁思敏	新疆医科大学第一附属医院呼吸科
高?辉	新疆医科大学第八附属医院布病科

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中文摘要:

目的?检测布鲁菌病（布病）急性期和慢性期患者治疗前后外周血CD19+CD24hiCD27+调节性B细胞（regulatory B cells, Bregs）和血清细胞因子IL-10和IFN-γ水平。分析布病患者不同病程中Bregs和细胞因子变化特点，评估机体免疫反应状态。方法?选取2021年6月—2022年7月在新疆医科大学第八附属医院诊治的急、慢性期布病患者各20例，在治疗前后分别抽取血标本，并设立健康对照组（20例），入组后抽取血标本。应用流式细胞术检测各组Bregs水平，ELISA法检测外周血IL-10和IFN-γ水平，对各组检测结果进行统计学分析。结果?与健康对照组相比，急、慢性期2组患者治疗前Bregs水平均明显升高；治疗后水平均下降，但慢性期组仍高于健康对照组和急性期组，差异均有统计学意义（P均＜0.05）。与健康对照组相比，治疗前急、慢性期组IL-10均明显升高，治疗后2组水平均下降，但仍高于健康对照组，且差异均具有统计学意义（P均＜0.05）。与对照组相比，治疗前，急、慢性期组血清IFN-γ水平均明显升高，治疗后，2组血清IFN-γ水平较治疗前明显下降，但急性期组仍高于健康对照组，差异有统计学意义（P＜0.05）。结论?布鲁杆菌感染机体后，伴随着高水平的IFN-γ机体炎症反应，急性期和慢性期均会出现Bregs、IL-10水平升高。急性期组治疗前炎症因子IFN-γ水平升高伴Bregs明显增加，治疗后明显下降。慢性期患者治疗后Bregs和IL-10水平仍偏高，提示出现并发症或转为慢性化可能。

英文摘要:

Objective　Detected the levels of CD19+CD24hiCD27+ regulatory B cells (Bregs) of peripheral blood and serum cytokines IL-10 and IFN-γ before and after treatment in patients with acute and chronic Brucellosis. The characteristics of Bregs and cytokines in different courses of brucellosis were analyzed, and the immune response status was evaluated. Method?Twenty patients with acute brucellosis and 20 patients with chronic brucellosis diagnosed in the Eighth Affiliated Hospital of Xinjiang Medical University were selected. Blood samples were taken before and after treatment, 20 healthy control population were set up. Flow cytometry was used to detect the level of Bregs in each group, the level of IL-10 and IFN-γ in peripheral blood were detected by ELISA, the results of each group were statistically analyzed. Result?Compared with healthy control group, the levels of Bregs were significantly increased before treatment in both acute and chronic groups, all decreased after treatment, the chronic stage group was still higher than the control group and acute stage group, the differences were statistically significant, P＜0.05. Compared with healthy control group, IL-10 was significantly increased in acute and chronic groups, the levels were still higher than those of the healthy control group after treatment, and the differences were statistically significant, P＜0.05. The serum IFN-γ level of acute and chronic Brucellosis groups were significantly increased before treatment, and significantly decreased in the 2 groups after treatment, but the acute stage group was still higher than the control group, the differences were statistically significant, P＜0.05. Conclusions?After the infection of Brucella, with a high level of IFN-γ, there is an increase in Bregs and IL-10 in both acute and chronic groupss The high levels of IFN-γ and Bregs in the acute group, decreased significantly after treatment. Patients with increased Bregs and IL-10 level after treatment in the chronic group suggested the probably occur of comorbidities and chronicity.

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