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| 结核分枝杆菌McSpoligotyping
基因分型在宜昌地区耐多药结核病中的应用研究
凝血筛查指标变化及其对临床分型的诊断价值 |
| Application of Mycobacterium tuberculosisgenotyping by McSpoligotyping in MDR-TB in Yichang |
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| DOI：10.3969/j.issn.1007-8134.2022.06.012 |
| 中文关键词: 结核分枝杆菌 北京基因型 熔解曲线 间隔区寡核苷酸分型法 分子流行病学 耐多药结核病 异烟肼 利福平 成簇炎 |
| 英文关键词: Mycobacterium tuberculosis Beijing genotype melting curve spacer oligonucleotide typing molecular epidemiology multidrug-resistant tuberculosis isoniazid rifampicin clustering |
| 基金项目:宜昌市医疗卫生科研项目（A19-301-48） |
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| 中文摘要: |
| [摘要] 目的 运用熔解曲线间隔区寡核苷酸分型（melting curve spacer oligonucleotide typing, McSpoligotyping）方法对宜昌地区耐多药结核分枝杆菌进行基因分型，以评价其在结核病流行病学调查中的应用价值。方法 选择2019年1月—2020年12月耐多药菌株77株作为实验组，并以异烟肼和利福平敏感菌株110株作为对照组，运用McSpoligotyping方法进行基因分型，将分型结果与SpolDB4.0数据库进行比对分析。结果 187株菌株中北京基因型116株（62.03％），非北京基因型71株（37.97％）。对照组110株中北京基因型60株（54.55％），其中数量最多的1组国际型别编码（spoligotype inter-national type, SIT）为1的北京基因型共44株（40.00％）；非北京基因型50株（45.45％），以T族、Manu族常见。实验组77株中北京基因型56株（72.73％），其中数量最多的1组为 SIT为1的北京基因型共44株（57.14％），非北京基因型21株（27.27％），以T族、Manu2族常见。实验组北京基因型菌株所占比例与对照组差异有统计学意义。实验组成簇率（79.22％）与对照组成簇率（79.09％）差异无统计学意义。结论 北京基因型菌株是宜昌地区结核病的主要流行菌株，在耐多药结核病患者中北京基因型菌株更为流行。耐多药结核菌株有一定的遗传多态性。McSpoligotyping方法操作简单、快速、对于结核病分子流行病学研究具有重要意义。 |
| 英文摘要: |
| [Abstract] Objective?To study the genotype multidrug-resistant Mycobacterium tuberculosis by melting curve spacer oligonucleotide method (McSpoligotyping) and evaluate its value in epidemiological investigation of tuberculosis in Yichang. Methods?Seventy-seven MDR-TB strains were selected from January 2019 to December 2020 as the experimental, and 110 HR-sensitive strains were selected as the control. Genotyping was performed using melting curve spacer oligonucleotide typing (McSpoligotyping), and the results were compared with the SpolDB4.0 database. Results?Of the 187 strains, 116 (62.03%) were Beijing genotype and 71 (37.97%) were non-Beijing genotype. Among the 110 strains in the control, 60 (54.55%) had Beijing genotype, of which the largest group was 44 (40.00%) with Beijing genotype with SIT of 1; 50 (45.45%) had non-Beijing genotype, with the T and Manu groups being common. Among the 77 strains in the experimental group, 56 (72.73%) were Beijing genotype, of which the largest group was 44 (57.14%) Beijing genotype with SIT of 1 and 21 (27.27%) non-Beijing strains, which were common in T and Manu2 families. The proportion of Beijing genotype strains in the experimental group was significantly different from the control (P ＜ 0.05). There was no significant difference in the cluster rate between the experimental (79.22%) and the control group (79.09%) (P ＞ 0.05). Conclusions?The Beijing genotype strains is the main prevalent strain of tuberculosis in the Yichangand tis more prevalent in MDR-TB patients. MDR-TB strains have some genetic polymorphism. The McSpoligotyping method is simple, fast and it is great significance for the molecular epidemiological studies of tuberculosis. |
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