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| 数字PCR和荧光定量PCR诊断急性期布鲁菌病的灵敏度比较初步研究 |
| A preliminary study on the sensitivity of digital PCR and fluorescence quantitative PCR in the diagnosis of acute brucellosis |
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| DOI：10.3969/j.issn.1007-8134.2019.04.006 |
| 中文关键词: 布鲁菌 数字PCR 荧光定量PCR 诊断 |
| 英文关键词: Brucella digital PCR fluorescent quantitative PCR diagnosis |
| 基金项目:北京市属医院科研培育计划（PX2016019，PX2019065）；首都医科大学附属北京地坛医院院内科研基金项目（DTQL201801） |
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| 中文摘要: |
| 目的 建立用于布鲁菌检测的数字PCR技术，初步用小样本开展研究，比较数字PCR和荧光定量PCR的灵敏度差异，验证数字PCR作为急性期布鲁菌病诊断技术的可行性。方法 选取符合我国急性期布鲁菌病诊断标准的20例患者的血清标本，分别用数字PCR和荧光定量PCR技术进行检测，初步比较2者在急性期布鲁菌病诊断中的灵敏度差异，并分析差异原因。结果 20例急性期布鲁菌病患者血清样本中，数字PCR检测阳性20例，荧光定量PCR检测阳性0例。结论 初步证实在急性期布鲁菌病患者血清标本检测中，数字PCR检测灵敏度高于荧光定量PCR，有良好的临床应用前景，但也存在一定局限性，尚须进一步扩大样本完成实验诊断技术临床研究，并进行多实验室验证。 |
| 英文摘要: |
| Objective A digital PCR technology for detection of Brucella was established. A preliminary study was carried out with small samples. To compared the sensitivity differences between digital PCR and fluorescence quantitative PCR and verify the feasibility of digital PCR as a diagnostic technique for acute brucellosis. Methods Serum samples from 20 patients who met the diagnostic criteria of acute brucellosis in China were detected by digital PCR and fluorescent quantitative PCR respectively. The sensitivity differences between the 2 methods in the diagnosis of acute brucellosis were preliminarily compared, and the reasons of the differences were analyzed. Results Among the 20 serum samples from patients with acute brucellosis, 20 were positive by digital PCR and 0 by fluorescent quantitative PCR. Conclusions It is preliminarily confirmed that the sensitivity of digital PCR is higher than that of fluorescent quantitative PCR in the detection of serum samples from patients with acute brucellosis. It has good clinical application prospects, but it also has some limitations. It is necessary to further expand the sample to complete the clinical research of experimental diagnosis and carry out multi-laboratory validation. |
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